Promega video about reporter genes assays:
https://www.youtube.com/watch?v=xqTudg1WdmY
Youtube video about how to make transgenic flies:
https://www.youtube.com/watch?v=1CJfj1GG_f8
Transgenic organisms are defined as a genetically modified organisms that contains DNA from two different genomes. A transgenic organism (or knock-in organism) contains a gene which is overexpressed for the purpose of ascertaining the gene function and location. The method of creating a transgenic organism is the same as creating a knock-out organism. A plasmid vector must be used for inserting the gene of interest into the genome. The vector is introduced into the gametic cells of the organism and the experssion is confirmed in the offspring.
“Make Transgenic Flies” will be completed first then do the “Use Transgenic Flies”.
Copy/past this link into your browser. Click on “Launch Interactive.” Complete the lab and answer the questions on the worksheet. Remember to read all the information.
https://www.biointeractive.org/classroom-resources/transgenic-fly-virtual-lab
25 days ago
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