Experimental Design for Biologists Reflection IV

(Ch. 23-24, 29-30, & 34)

 Instructions: Read Ch. 22 only pg 137-146 & 152-154, Read Ch. 23 only pg. 155-162. (I suggest you skim the other examples and read them if they seem relevant to your project.) Read all of CH. 29, and then read through the very short chapters 30 & 34. Use those last two chapters as a quick reference guide for your project.  Be prepared to discuss these concepts in class, as they relate to your project.

1.     Briefly explain the purpose of each treatment from the caffeine experiment to the right (explain more than just e.g. “negative control” by describing the purpose of that particular control):

a.      

b.       

c.        

d.       

e.        

f.        

g.       

2.     Answer the following about replication:

a.     Explain the difference between a biological replicate, a technical replicate,  and an experimental replicate.

b.     Provide an example of each type of replicate for the hypothetical caffeine/blood pressure experiment explained earlier.

3.     Describe two benefits provided by each of the following:

a.     Time course experiments:

b.     Dose response experiments:

4. Briefly describe a negative control, positive control, biological replicate, and technical replicate for your proposed study. If you haven’t gotten to the point to be able to do this, then use the hypothetical experiment from the assignment last week regarding the question “How does cricket respiration rate change with increasing temperature?”