Write 

Objective, Background, method, result and conclusion (ALL IN PAST TENS )

ANSWER THE Questions.

SEE THE RESULTS IN EXCEL SHEET

• a table of the data from the growth experiment 
  • Time in mins
  • count date from each group. 
  • average data 
  • actual dilution (remember the 0.1 ml added to the plate is another 10 fold dilution)
  • Cfu/ml (represent with one digit to the left of the decimal point and two to the right then the exponent)
  • Absorbance at 600nm 
  • Calculated CFU using the Aligent website 
• GraphTitleY axisX axisE coli Growth CurveAbsorbance (600nm)Time (mins)E. coli Growth CurveLog bacterial numbers (CFU MM/ml) Aligent)TimeE. coli Growth CurveLog bacterial numbers (CFU MM/ml)TimeE coli Standard CurveAbsorbance (600nm)Cell numbers (MM/ml) for both Aligent and your CFU data
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Questions

• Look at the growth curves using Aligent CFU and your CFU: are they similar or different.  If they are different why is that..
• Look at the aligent  plate count vs absorbance graph  – are they similar are they different.  If they are different why do you think that 
• Look at the aligent and your cfu data vs time  – calculate the maximum time of doubling 
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