Organic Chemistry Identifying Unknown Analgesics Lab Experiment Questions

Experiment 3: Identifying Unknown AnalgesicsSafety Alert:
x
Wear PPE (safety goggles, gloves, and lab coat) while performing the
experiments.
x
Perform all experiments in a hood.
x
Organic compounds are much more rapidly absorbed through the skin when they are in
solution, particularly in water-soluble solvents such as acetone and ethanol. For this
reason, do not rinse organic materials off your skin with solvents such as acetone; wash
your hands thoroughly with soap and warm water instead.
Material lists
Test tubes (10 mL) and test tube rack
Conical Vials
Pestle
Disposable graduated transfer pipettes
Centrifuge
Disposable glass pipet
Round-bottom flask
Melting point apparatus
Alumina
MeOH
Amazon Basic Care Extra Strength Pain Relief, Acetaminophen Caplets, 500 mg
Amazon Basic Care Aspirin Pain Reliever and Fever Reducer (NSAID), 325 mg
Major Pharmaceuticals Ibuprophen 200mg
CVS Health Naproxen Sodium Caplets 220 mg
Purpose:
x
Identify four unknown analgesics based on their difference in acidity, polarity and melting
point.
x
Know how to utilize pKa value to predict whether an acid-base reaction will take place.
Substrates and Reaction Equations:
O
O
HN
O
O
O
O
OH
O
OH
ONa
OH
acetaminophen
(Tylenol)
acetylsalicyclic acid
(Aspirin)
Organic Acid + NaHCO3
pKa = ?
ibuprofen
(Advil, Motrin)
Conjugate Base
Base
naproxen sodium
(Aleve)
+
H2CO3
pKa 6.4
Conjugated Acid
CO2 + H2O
Reagent Table (Please complete before the lab starts):
Chemical Name
Functional
groups
present in
the molecule
M.P.
(°C)
B.P.
(°C)
Acetaminophen
N/A
Aspirin
N/A
Ibuprofen
N/A
Naproxen Sodium
N/A
MeOH
N/A
Is it an
organic
acid?
pKa if it
is an
organic
acid
N/A
N/A
Hazards/Precautions
You will work in a group of four for this experiment. Each student will be assigned one of
he f
nkn n analge ic . Be
e
ec d all b e a i n incl ding
eamma e
result if the table includes all four unknown.
Procedures:
¾ Predict an Acid-Base Reaction:
x Given the pKa of H2CO3 is 6.4, determine whether the acid-base reaction between a
given analgesic and NaHCO3 will occur or not (Fill in the following table as part of prelab exercise).
Name
Is it an organic
acid?
pKa of the
organic acid
Will it react with
NaHCO3?
Acetaminophen
Aspirin
Ibuprofen
Naproxen Sodium
x
Transfer 3 mL of saturated NaHCO3 each to four scintillated vials and drop1 tablet of
each of the unknown analgesic into each of the four vials with the saturated NaHCO3
solution at the same time. Observe if there is any bubbling in the test tube. Record your
observations below and draw your conclusion.
Unknown
Observations
Conclusion and explanations
Analgesic #
1
2
3
4
¾ Extraction of active ingredients from the tablet:
x
You will be assigned one of the four unknown analgesics
x
Take 1 tablet your assigned analgesic and grind between two sheets of weighing
paper.
x
Add to a 3 mL conical vial, add 2 mL methanol, cap and shake. Allow the solid to
settle; use a pipette to transfer the liquid to a centrifuge tube.
x
Repeat the methanol extraction two additional times and combine the methanol
extracts.
x
Centrifuge the mixture for 3 minutes (Make sure to balance the tubes using another
centrifuge tube of similar weight!)
¾ pH of Unknown Analgesic
x
Using a TLC spotter to transfer a small portion of your unknown analgesics in MeOH
onto blue litmus paper* and record your observation in Table 2. If there is no obvious
color change, take a new TLC spotter to transfer another small portion of your
unknown analgesics in MeOH onto a pink litmus paper* and record your observation
in the table below.
Unknown
Analgesic #
Upon contact with blue litmus paper
Observations
conclusion
Upon contact with pink litmus paper
observation
conclusion
1
2
3
4
*Please note blue litmus paper turns red under acidic conditions and red litmus paper turns blue under basic (i.e.
alkaline) conditions.
¾ Thin-layer Chromatography
x
Prepare your TLC chamber by adding 3 mL of ethyl acetate in it. Place a piece of
filter paper inside the chamber to saturate the air in the chamber with the solvent
vapors. Keep the cap closed.
x
Obtain two silica gel TLC plates from your instructor. Be careful not to bend the plate
because the flexible backing may cause some of the silica gel to flake off.
x
Take a pencil and draw a line with three equally spaced tick marks about 10 mm from
the bottom (This will be your baseline) and another line about 10 mm from the top
(This will be your solvent front line) for each TLC plate. These marks will eventually
become spotted with different samples, so label them accordingly: Use a pencil and
write U, Ace, Asp (representing Unknown, Aspirin, and Acetaminophen) above the
top pencil line on the first TLC plate, and write U, Ibu, and Nap ((representing
Unknown, Ibuprofen, and Naproxen Sodium) above the top pencil line on the second
TLC plate.
x
Using a TLC spotter, draw up a solution containing your unknown and spot it lightly
on both plates at the left tick marks. Allow the solvent to evaporate. Repeat for the
four known analgesic standards, in the right and middle tick marks of the two TLC
plates according to the writings above the top pencil lines of each plate.
x
Since all of these samples are UV active, check to make sure you have enough
analyte on the plate by looking at the plate under UV light. You should see a
black/blue spot on each tick mark.
x
Place one TLC plate into the chamber, and make sure that the solvent level is below
the baseline of your plate.
x
Develop the plate until the solvent front reaches the top pencil line. Take the plate
immediately out and allow the solvent to evaporate.
x
Place your plate under UV light. Use a pencil and draw a circle around each of the
spots you observe.
x
Repeat the process with the second TLC plate.
x
Calculate the retention factor (Rf) of each of the samples (Rf = danalyte/dsolvent front) and
complete the table below.
Analyte
Distance traveled
by analyte
Unknown #
Plate 1
Acetaminophen
Standard
Plate 2
Distant
travel by
solvent front
Rf Value
Plate 1
Conclusions and
explain
Plate 2
Average
Aspirin Standard
Ibuprofen
Standard
Naproxen Sodium
Standard
¾ Melting Point and Percent of Recovery
x
Evaporate the methanol using the rotary evaporator.
x
Weigh the flask, determine melting point range and percent of recovery.
Unknown
Analgesic
#
Mass of your
empty roundbottom flask
Mass of roundbottom flask with
active ingredient
Mass of
active
ingredient
m.p. and
conclusion
(Explain)
Percent of recovery
(Show your work)
Conclusion and Discussions:
(Briefly summarize this experiment, report your observation and results, draw conclusions and
offer explanations).
SAFETY DATA SHEET
Version 6.8
Revision Date 05/25/2021
Print Date 08/14/2021
SECTION 1: Identification of the substance/mixture and of the company/undertaking
1.1
1.2
Product identifiers
Product name
:
Methanol
Product Number
Brand
Index-No.
CAS-No.
:
:
:
:
34860
SIGALD
603-001-00-X
67-56-1
Relevant identified uses of the substance or mixture and uses advised against
Identified uses
1.3
1.4
: Laboratory chemicals, Synthesis of substances
Details of the supplier of the safety data sheet
Company
: Sigma-Aldrich Inc.
3050 SPRUCE ST
ST. LOUIS MO 63103
UNITED STATES
Telephone
Fax
: +1 314 771-5765
: +1 800 325-5052
Emergency telephone
Emergency Phone #
: 800-424-9300 CHEMTREC (USA) +1-703527-3887 CHEMTREC (International) 24
Hours/day; 7 Days/week
SECTION 2: Hazards identification
2.1
Classification of the substance or mixture
GHS Classification in accordance with 29 CFR 1910 (OSHA HCS)
Flammable liquids (Category 2), H225
Acute toxicity, Oral (Category 3), H301
Acute toxicity, Inhalation (Category 3), H331
Acute toxicity, Dermal (Category 3), H311
Specific target organ toxicity – single exposure (Category 1), Eyes, Central nervous system,
H370
For the full text of the H-Statements mentioned in this Section, see Section 16.
2.2
GHS Label elements, including precautionary statements
Pictogram
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operates as MilliporeSigma in the US and Canada
Page 1 of 13
Signal word
Danger
Hazard statement(s)
H225
H301 + H311 + H331
H370
Highly flammable liquid and vapor.
Toxic if swallowed, in contact with skin or if inhaled.
Causes damage to organs (Eyes, Central nervous system).
Precautionary statement(s)
P210
Keep away from heat/ sparks/ open flames/ hot surfaces. No
smoking.
P233
Keep container tightly closed.
P240
Ground/bond container and receiving equipment.
P241
Use explosion-proof electrical/ ventilating/ lighting/ equipment.
P242
Use only non-sparking tools.
P243
Take precautionary measures against static discharge.
P260
Do not breathe dust/ fume/ gas/ mist/ vapors/ spray.
P264
Wash skin thoroughly after handling.
P270
Do not eat, drink or smoke when using this product.
P271
Use only outdoors or in a well-ventilated area.
P280
Wear protective gloves/ eye protection/ face protection.
P301 + P310 + P330
IF SWALLOWED: Immediately call a POISON CENTER/ doctor.
Rinse mouth.
P303 + P361 + P353
IF ON SKIN (or hair): Take off immediately all contaminated
clothing. Rinse skin with water/ shower.
P304 + P340 + P311
IF INHALED: Remove person to fresh air and keep comfortable
for breathing. Call a POISON CENTER/ doctor.
P307 + P311
IF exposed: Call a POISON CENTER or doctor/ physician.
P362
Take off contaminated clothing and wash before reuse.
P370 + P378
In case of fire: Use dry sand, dry chemical or alcohol-resistant
foam to extinguish.
P403 + P233
Store in a well-ventilated place. Keep container tightly closed.
P403 + P235
Store in a well-ventilated place. Keep cool.
P405
Store locked up.
P501
Dispose of contents/ container to an approved waste disposal
plant.
2.3
Hazards not otherwise classified (HNOC) or not covered by GHS – none
SECTION 3: Composition/information on ingredients
3.1
Substances
Synonyms
Formula
Molecular weight
CAS-No.
EC-No.
Index-No.
Component
: Methyl alcohol
: CH4O
: 32.04 g/mol
: 67-56-1
: 200-659-6
: 603-001-00-X
Classification
Concentration
Methanol
Flam. Liq. 2; Acute Tox. 3; = 10 %: STOT SE 1,
H370; 3 – < 10 %: STOT SE 2, H371; For the full text of the H-Statements mentioned in this Section, see Section 16. SECTION 4: First aid measures 4.1 Description of first-aid measures General advice First aiders need to protect themselves. Show this material safety data sheet to the doctor in attendance. If inhaled After inhalation: fresh air. Immediately call in physician. If breathing stops: immediately apply artificial respiration, if necessary also oxygen. In case of skin contact In case of skin contact: Take off immediately all contaminated clothing. Rinse skin with water/ shower. Call a physician immediately. In case of eye contact After eye contact: rinse out with plenty of water. Call in ophthalmologist. Remove contact lenses. If swallowed After swallowing: fresh air. Make victim drink ethanol (e.g. 1 drinking glass of a 40% alcoholic beverage). Call a doctor immediately (mention methanol ingestion). Only in exceptional cases, if no medical care is available within one hour, induce vomiting (only in fully conscious persons) and make victim drink ethanol again (approx. 0.3 ml of a 40% alcoholic beverage/kg body weight/hour). 4.2 Most important symptoms and effects, both acute and delayed The most important known symptoms and effects are described in the labelling (see section 2.2) and/or in section 11 4.3 Indication of any immediate medical attention and special treatment needed No data available SECTION 5: Firefighting measures 5.1 Extinguishing media Suitable extinguishing media Water Foam Carbon dioxide (CO2) Dry powder Unsuitable extinguishing media For this substance/mixture no limitations of extinguishing agents are given. 5.2 Special hazards arising from the substance or mixture Carbon oxides Combustible. Pay attention to flashback. Vapors are heavier than air and may spread along floors. SIGALD - 34860 The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the US and Canada Page 3 of 13 Development of hazardous combustion gases or vapours possible in the event of fire. Forms explosive mixtures with air at ambient temperatures. 5.3 Advice for firefighters Stay in danger area only with self-contained breathing apparatus. Prevent skin contact by keeping a safe distance or by wearing suitable protective clothing. 5.4 Further information Remove container from danger zone and cool with water. Prevent fire extinguishing water from contaminating surface water or the ground water system. SECTION 6: Accidental release measures 6.1 Personal precautions, protective equipment and emergency procedures Advice for non-emergency personnel: Do not breathe vapors, aerosols. Avoid substance contact. Ensure adequate ventilation. Keep away from heat and sources of ignition. Evacuate the danger area, observe emergency procedures, consult an expert. For personal protection see section 8. 6.2 Environmental precautions Do not let product enter drains. Risk of explosion. 6.3 Methods and materials for containment and cleaning up Cover drains. Collect, bind, and pump off spills. Observe possible material restrictions (see sections 7 and 10). Take up carefully with liquid-absorbent material (e.g. Chemizorb®). Dispose of properly. Clean up affected area. 6.4 Reference to other sections For disposal see section 13. SECTION 7: Handling and storage 7.1 Precautions for safe handling Advice on safe handling Work under hood. Do not inhale substance/mixture. Avoid generation of vapours/aerosols. Advice on protection against fire and explosion Keep away from open flames, hot surfaces and sources of ignition.Take precautionary measures against static discharge. Hygiene measures Immediately change contaminated clothing. Apply preventive skin protection. Wash hands and face after working with substance. For precautions see section 2.2. 7.2 Conditions for safe storage, including any incompatibilities Storage conditions Keep container tightly closed in a dry and well-ventilated place. Keep away from heat and sources of ignition. Keep locked up or in an area accessible only to qualified or authorized persons. Storage class (TRGS 510): 3: Flammable liquids 7.3 Specific end use(s) Apart from the uses mentioned in section 1.2 no other specific uses are stipulated SIGALD - 34860 The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the US and Canada Page 4 of 13 SECTION 8: Exposure controls/personal protection 8.1 Control parameters Ingredients with workplace control parameters Component CAS-No. Value Control parameters Methanol 67-56-1 TWA 200 ppm Remarks USA. ACGIH Threshold Limit Values (TLV) Danger of cutaneous absorption STEL 250 ppm USA. ACGIH Threshold Limit Values (TLV) Danger of cutaneous absorption TWA 200 ppm USA. NIOSH Recommended 260 mg/m3 Exposure Limits Potential for dermal absorption ST 250 ppm USA. NIOSH Recommended 325 mg/m3 Exposure Limits Potential for dermal absorption TWA 200 ppm USA. Occupational Exposure 260 mg/m3 Limits (OSHA) - Table Z-1 Limits for Air Contaminants STEL 250 ppm USA. OSHA - TABLE Z-1 Limits 325 mg/m3 for Air Contaminants 1910.1000 Skin notation TWA 200 ppm USA. OSHA - TABLE Z-1 Limits 260 mg/m3 for Air Contaminants 1910.1000 Skin notation C 1,000 ppm California permissible exposure limits for chemical contaminants (Title 8, Article 107) Skin PEL 200 ppm California permissible exposure 260 mg/m3 limits for chemical contaminants (Title 8, Article 107) Skin STEL 250 ppm California permissible exposure 325 mg/m3 limits for chemical contaminants (Title 8, Article 107) Skin Biological occupational exposure limits Component CAS-No. Parameters Value Methanol 15 mg/l 67-56-1 Basis Methanol SIGALD - 34860 The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the US and Canada Biological specimen Urine Basis ACGIH Biological Exposure Indices (BEI) Page 5 of 13 Remarks End of shift (As soon as possible after exposure ceases) Derived No Effect Level (DNEL) Application Area Routes of Health effect exposure Value Workers Consumers Consumers Workers Consumers Consumers Workers Workers Workers Workers Consumers Consumers Consumers Consumers 40mg/kg BW/d 8mg/kg BW/d 8mg/kg BW/d 40mg/kg BW/d 8mg/kg BW/d 8mg/kg BW/d 260 mg/m3 260 mg/m3 260 mg/m3 260 mg/m3 50 mg/m3 50 mg/m3 50 mg/m3 50 mg/m3 Skin contact Skin contact Ingestion Skin contact Skin contact Ingestion Inhalation Inhalation Inhalation Inhalation Inhalation Inhalation Inhalation Inhalation Long-term systemic effects Long-term systemic effects Long-term systemic effects Acute systemic effects Acute systemic effects Acute systemic effects Acute systemic effects Acute local effects Long-term systemic effects Long-term local effects Acute systemic effects Acute local effects Long-term systemic effects Long-term local effects Predicted No Effect Concentration (PNEC) Compartment Value Soil Sea water Fresh water Fresh water sediment Onsite sewage treatment plant 8.2 23.5 mg/kg 15.4 mg/l 154 mg/l 570.4 mg/kg 100 mg/kg Exposure controls Appropriate engineering controls Immediately change contaminated clothing. Apply preventive skin protection. Wash hands and face after working with substance. Personal protective equipment Eye/face protection Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166(EU). Safety glasses Skin protection This recommendation applies only to the product stated in the safety data sheet, supplied by us and for the designated use. When dissolving in or mixing with other substances and under conditions deviating from those stated in EN374 please contact the supplier of CE-approved gloves (e.g. KCL GmbH, D-36124 Eichenzell, Internet: www.kcl.de). Full contact Material: butyl-rubber Minimum layer thickness: 0.7 mm Break through time: 480 min Material tested:Butoject® (KCL 898) This recommendation applies only to the product stated in the safety data sheet, supplied by us and for the designated use. When dissolving in or mixing with other substances and under conditions deviating from those stated in EN374 please SIGALD - 34860 The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the US and Canada Page 6 of 13 contact the supplier of CE-approved gloves (e.g. KCL GmbH, D-36124 Eichenzell, Internet: www.kcl.de). Splash contact Material: Viton® Minimum layer thickness: 0.7 mm Break through time: 120 min Material tested:Vitoject® (KCL 890 / Aldrich Z677698, Size M) Body Protection Flame retardant antistatic protective clothing. Respiratory protection required when vapours/aerosols are generated. Our recommendations on filtering respiratory protection are based on the following standards: DIN EN 143, DIN 14387 and other accompanying standards relating to the used respiratory protection system. Control of environmental exposure Do not let product enter drains. Risk of explosion. SECTION 9: Physical and chemical properties 9.1 Information on basic physical and chemical properties a) Appearance Form: liquid Color: colorless b) Odor characteristic c) Odor Threshold 10 ppm d) pH No data available e) Melting point/freezing point Melting point/range: -98 °C (-144 °F) f) Initial boiling point and boiling range 64.7 °C 148.5 °F g) Flash point 9.7 °C (49.5 °F) - closed cup - Regulation (EC) No. 440/2008, Annex, A.9 h) Evaporation rate 6.3 - Diethyl ether1.9 - n-butyl acetate i) Flammability (solid, gas) No data available j) Upper/lower flammability or explosive limits Upper explosion limit: 44 %(V) Lower explosion limit: 5.5 %(V) k) Vapor pressure 169.27 hPa at 25 °C (77 °F) l) Vapor density 1.11 m) Relative density 0.79 - 0.8 at 20 °C (68 °F) n) Water solubility 1,000 g/l at 20 °C (68 °F) - completely misciblesoluble o) Partition coefficient: n-octanol/water log Pow: -0.77 - (Lit.), Bioaccumulation is not expected. p) Autoignition temperature 455.0 °C (851.0 °F) at 1,013 hPa - DIN 51794 SIGALD - 34860 The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the US and Canada Page 7 of 13 9.2 q) Decomposition temperature Distillable in an undecomposed state at normal pressure. r) Viscosity 0.54 - 0.59 mm2/s at 20 °C (68 °F) - s) Explosive properties No data available t) Oxidizing properties No data available Other safety information Minimum ignition energy 0.14 mJ Conductivity < 1 µS/cm Relative vapor density 1.11 SECTION 10: Stability and reactivity 10.1 Reactivity Vapors may form explosive mixture with air. 10.2 Chemical stability The product is chemically stable under standard ambient conditions (room temperature) . 10.3 Possibility of hazardous reactions Risk of explosion with: Oxidizing agents perchloric acid perchlorates salts of oxyhalogenic acids chromium(VI) oxide halogen oxides nitrogen oxides nonmetallic oxides chromosulfuric acid chlorates hydrides zinc diethyl halogens powdered magnesium hydrogen peroxide Nitric acid sulfuric acid permanganic acid sodium hypochlorite Exothermic reaction with: acid halides Acid anhydrides Reducing agents acids Bromine Chlorine Chloroform magnesium tetrachloromethane SIGALD - 34860 The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the US and Canada Page 8 of 13 Risk of ignition or formation of inflammable gases or vapours with: Fluorine Oxides of phosphorus Raney-nickel Generates dangerous gases or fumes in contact with: Alkaline earth metals Alkali metals 10.4 Conditions to avoid Warming. 10.5 Incompatible materials various plastics, magnesium, zinc alloys 10.6 Hazardous decomposition products In the event of fire: see section 5 SECTION 11: Toxicological information 11.1 Information on toxicological effects Acute toxicity Acute toxicity estimate Oral - 100.1 mg/kg (Expert judgment) Symptoms: Nausea, Vomiting Acute toxicity estimate Inhalation - 4 h - 3.1 mg/l (Expert judgment) Symptoms: Irritation symptoms in the respiratory tract. Acute toxicity estimate Dermal - 300.1 mg/kg (Expert judgment) Skin corrosion/irritation Skin - Rabbit Result: No skin irritation Remarks: (ECHA) Drying-out effect resulting in rough and chapped skin. Serious eye damage/eye irritation Eyes - Rabbit Result: No eye irritation Remarks: (ECHA) Respiratory or skin sensitization Sensitisation test: - Guinea pig Result: negative (OECD Test Guideline 406) Germ cell mutagenicity Based on available data the classification criteria are not met. Test Type: Ames test Test system: Salmonella typhimurium Metabolic activation: with and without metabolic activation Method: OECD Test Guideline 471 Result: negative Test Type: In vitro mammalian cell gene mutation test Test system: Chinese hamster lung cells SIGALD - 34860 The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the US and Canada Page 9 of 13 Metabolic activation: with and without metabolic activation Method: OECD Test Guideline 476 Result: negative Test Type: Micronucleus test Species: Mouse Cell type: Bone marrow Application Route: Intraperitoneal injection Method: OECD Test Guideline 474 Result: negative Carcinogenicity Did not show carcinogenic effects in animal experiments. IARC: No ingredient of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC. NTP: No ingredient of this product present at levels greater than or equal to 0.1% is identified as a known or anticipated carcinogen by NTP. OSHA: No component of this product present at levels greater than or equal to 0.1% is on OSHA’s list of regulated carcinogens. Reproductive toxicity Based on available data the classification criteria are not met. Specific target organ toxicity - single exposure Causes damage to organs. - Eyes, Central nervous system Remarks: Classified according to Regulation (EU) 1272/2008, Annex VI (Table 3.1/3.2) Specific target organ toxicity - repeated exposure No data available Aspiration hazard No data available 11.2 Additional Information RTECS: PC1400000 To the best of our knowledge, the chemical, physical, and toxicological properties have not been thoroughly investigated. Systemic effects: acidosis drop in blood pressure agitation, spasms inebriation Dizziness Drowsiness Headache Impairment of vision Blindness narcosis Coma Symptoms may be delayed. Damage to: SIGALD - 34860 The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the US and Canada Page 10 of 13 Liver Kidney Cardiac Irreversible damage of the optical nerve. Other dangerous properties can not be excluded. This substance should be handled with particular care. Stomach - Irregularities - Based on Human Evidence Stomach - Irregularities - Based on Human Evidence SECTION 12: Ecological information 12.1 Toxicity Toxicity to fish flow-through test LC50 - Lepomis macrochirus (Bluegill) - 15,400.0 mg/l - 96 h (US-EPA) Toxicity to daphnia and other aquatic invertebrates semi-static test EC50 - Daphnia magna (Water flea) - 18,260 mg/l 96 h (OECD Test Guideline 202) Toxicity to algae static test ErC50 - Pseudokirchneriella subcapitata (green algae) - ca. 22,000.0 mg/l - 96 h (OECD Test Guideline 201) Toxicity to bacteria static test IC50 - activated sludge - > 1,000 mg/l – 3 h
(OECD Test Guideline 209)
12.2 Persistence and degradability
Biodegradability
Result: 99 % – Readily biodegradable.
(OECD Test Guideline 301D)
Biochemical Oxygen
Demand (BOD)
600 – 1,120 mg/g
Remarks: (IUCLID)
Chemical Oxygen
Demand (COD)
1,420 mg/g
Remarks: (IUCLID)
Theoretical oxygen
demand
1,500 mg/g
Remarks: (Lit.)
Ratio BOD/ThBOD
76 %
Remarks: Closed Bottle test(IUCLID)
12.3 Bioaccumulative potential
Bioaccumulation
Cyprinus carpio (Carp) – 72 d
at 20 °C – 5 mg/l(Methanol)
Bioconcentration factor (BCF): 1.0
12.4 Mobility in soil
Will not adsorb on soil.
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Page 11 of 13
12.5 Results of PBT and vPvB assessment
PBT/vPvB assessment not available as chemical safety assessment not required/not
conducted
12.6 Other adverse effects
Additional ecological
information
Avoid release to the environment.
Stability in water
at 19 °C83 – 91 % – 72 h
Remarks: Hydrolyzes on contact with water.Hydrolyzes readily.
SECTION 13: Disposal considerations
13.1 Waste treatment methods
Product
Waste material must be disposed of in accordance with the national and local regulations.
Leave chemicals in original containers. No mixing with other waste. Handle uncleaned
containers like the product itself. See www.retrologistik.com for processes regarding the
return of chemicals and containers, or contact us there if you have further questions.
SECTION 14: Transport information
DOT (US)
UN number: 1230 Class: 3
Proper shipping name: Methanol
Reportable Quantity (RQ): 5000 lbs
Poison Inhalation Hazard: No
Packing group: II
IMDG
UN number: 1230 Class: 3 (6.1)
Proper shipping name: METHANOL
Packing group: II
IATA
UN number: 1230 Class: 3 (6.1)
Proper shipping name: Methanol
Packing group: II
EMS-No: F-E, S-D
SECTION 15: Regulatory information
SARA 302 Components
This material does not contain any components with a section 302 EHS TPQ.
SARA 313 Components
The following components are subject to reporting levels established by SARA Title III,
Section 313:
CAS-No.
Revision Date
Methanol
67-56-1
2007-07-01
SARA 311/312 Hazards
Fire Hazard, Acute Health Hazard, Chronic Health Hazard
Massachusetts Right To Know Components
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operates as MilliporeSigma in the US and Canada
Page 12 of 13
No components are subject to the Massachusetts Right to Know Act.
SECTION 16: Other information
Further information
The above information is believed to be correct but does not purport to be all inclusive
and shall be used only as a guide. The information in this document is based on the
present state of our knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any guarantee of the properties of
the product. Sigma-Aldrich Corporation and its Affiliates shall not be held liable for any
damage resulting from handling or from contact with the above product. See
www.sigma-aldrich.com and/or the reverse side of invoice or packing slip for additional
terms and conditions of sale.
Copyright 2020 Sigma-Aldrich Co. LLC. License granted to make unlimited paper copies
for internal use only.
The branding on the header and/or footer of this document may temporarily not visually
match the product purchased as we transition our branding. However, all of the
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Version: 6.8
Revision Date: 05/25/2021
SIGALD – 34860
The life science business of Merck KGaA, Darmstadt, Germany
operates as MilliporeSigma in the US and Canada
Print Date: 08/14/2021
Page 13 of 13
SAFETY DATA SHEET
Version 6.3
Revision Date 08/12/2021
Print Date 08/14/2021
SECTION 1: Identification of the substance/mixture and of the company/undertaking
1.1
1.2
Product identifiers
:
Product Number
Brand
CAS-No.
: A2093
: Sigma
: 50-78-2
Relevant identified uses of the substance or mixture and uses advised against
Identified uses
1.3
1.4
Aspirin
Product name
: Laboratory chemicals, Synthesis of substances
Details of the supplier of the safety data sheet
Company
: Sigma-Aldrich Inc.
3050 SPRUCE ST
ST. LOUIS MO 63103
UNITED STATES
Telephone
Fax
: +1 314 771-5765
: +1 800 325-5052
Emergency telephone
Emergency Phone #
: 800-424-9300 CHEMTREC (USA) +1-703527-3887 CHEMTREC (International) 24
Hours/day; 7 Days/week
SECTION 2: Hazards identification
2.1
Classification of the substance or mixture
GHS Classification in accordance with 29 CFR 1910 (OSHA HCS)
Acute toxicity, Oral (Category 4), H302
Short-term (acute) aquatic hazard (Category 3), H402
For the full text of the H-Statements mentioned in this Section, see Section 16.
2.2
GHS Label elements, including precautionary statements
Pictogram
Signal word
Warning
Hazard statement(s)
H302
H402
Harmful if swallowed.
Harmful to aquatic life.
Sigma – A2093
The life science business of Merck KGaA, Darmstadt, Germany
operates as MilliporeSigma in the US and Canada
Page 1 of 9
Precautionary statement(s)
P264
Wash skin thoroughly after handling.
P270
Do not eat, drink or smoke when using this product.
P273
Avoid release to the environment.
P301 + P312 + P330
IF SWALLOWED: Call a POISON CENTER/ doctor if you feel
unwell. Rinse mouth.
P501
Dispose of contents/ container to an approved waste disposal
plant.
2.3
Hazards not otherwise classified (HNOC) or not covered by GHS – none
SECTION 3: Composition/information on ingredients
3.1
Substances
Synonyms
Formula
Molecular weight
CAS-No.
EC-No.
: ASA
O-Acetylsalicylic acid
2-Acetoxybenzoic acid
Acetylsalicylic acid
Aspirin
:
:
:
:
C9H8O4
180.16 g/mol
50-78-2
200-064-1
Component
Classification
Concentration
Acute Tox. 4; Aquatic
Acute 3; H302, H402
10,000 mg/kg
(OECD Test Guideline 401)
LC50 Inhalation – Rat – male and female – 4 h – > 2.3 mg/l
(OECD Test Guideline 403)
Dermal: No data available
No data available
Skin corrosion/irritation
Skin – Rabbit
Result: No skin irritation – 24 h
(OECD Test Guideline 404)
Serious eye damage/eye irritation
Eyes – Rabbit
Result: No eye irritation
(OECD Test Guideline 405)
Respiratory or skin sensitisation
Draize Test – Guinea pig
Result: Does not cause skin sensitisation.
– Mouse
Result: Does not cause respiratory sensitisation.
Germ cell mutagenicity
No data available
Aldrich – GF18024511
The life science business of Merck KGaA, Darmstadt, Germany
operates as MilliporeSigma in the US and Canada
Page 6 of 9
Ames test
Bacillus subtilis
Result: negative
(IUCLID)
Carcinogenicity
IARC:
No component of this product present at levels greater than or equal to 0.1% is
identified as probable, possible or confirmed human carcinogen by IARC.
NTP:
No component of this product present at levels greater than or equal to 0.1% is
identified as a known or anticipated carcinogen by NTP.
OSHA:
No component of this product present at levels greater than or equal to 0.1% is
on OSHA’s list of regulated carcinogens.
Reproductive toxicity
No data available
Specific target organ toxicity – single exposure
No data available
Specific target organ toxicity – repeated exposure
No data available
Aspiration hazard
No data available
Additional Information
RTECS: Not available
Cough, chest pain, Difficulty in breathing, Gastrointestinal disturbance
To the best of our knowledge, the chemical, physical, and toxicological properties have not
been thoroughly investigated.
Liver – Irregularities – Based on Human Evidence
Liver – Irregularities – Based on Human Evidence
SECTION 12: Ecological information
12.1 Toxicity
No data available
12.2 Persistence and degradability
The methods for determining the biological degradability are not applicable to inorganic
substances.
12.3 Bioaccumulative potential
No data available
12.4 Mobility in soil
No data available
12.5 Results of PBT and vPvB assessment
PBT/vPvB assessment not available as chemical safety assessment not required/not
conducted
Aldrich – GF18024511
The life science business of Merck KGaA, Darmstadt, Germany
operates as MilliporeSigma in the US and Canada
Page 7 of 9
12.6 Other adverse effects
No ecological problems are to be expected when the product is handled and used with due
care and attention.
SECTION 13: Disposal considerations
13.1 Waste treatment methods
Product
Offer surplus and non-recyclable solutions to a licensed disposal company.
Contaminated packaging
Dispose of as unused product.
SECTION 14: Transport information
DOT (US)
Not dangerous goods
IMDG
Not dangerous goods
IATA
Not dangerous goods
SECTION 15: Regulatory information
SARA 302 Components
No chemicals in this material are subject to the reporting requirements of SARA Title III,
Section 302.
SARA 313 Components
The following components are subject to reporting levels established by SARA Title III,
Section 313:
CAS-No.
Revision Date
Aluminium oxide
1344-28-1
1994-04-01
SARA 311/312 Hazards
Chronic Health Hazard
Massachusetts Right To Know Components
Aluminium oxide
Pennsylvania Right To Know Components
Aluminium oxide
Aluminium oxide
Aldrich – GF18024511
The life science business of Merck KGaA, Darmstadt, Germany
operates as MilliporeSigma in the US and Canada
CAS-No.
1344-28-1
Revision Date
1994-04-01
CAS-No.
1344-28-1
Revision Date
1994-04-01
CAS-No.
Revision Date
Page 8 of 9
New Jersey Right To Know Components
Aluminium oxide
1344-28-1
1994-04-01
CAS-No.
1344-28-1
Revision Date
1994-04-01
California Prop. 65 Components
This product does not contain any chemicals known to State of California to cause cancer,
birth defects, or any other reproductive harm.
SECTION 16: Other information
Further information
Copyright 2018 Sigma-Aldrich Co. LLC. License granted to make unlimited paper copies
for internal use only.
The above information is believed to be correct but does not purport to be all inclusive
and shall be used only as a guide. The information in this document is based on the
present state of our knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any guarantee of the properties of
the product. Sigma-Aldrich Corporation and its Affiliates shall not be held liable for any
damage resulting from handling or from contact with the above product. See
www.sigma-aldrich.com and/or the reverse side of invoice or packing slip for additional
terms and conditions of sale.
The branding on the header and/or footer of this document may temporarily not visually
match the product purchased as we transition our branding. However, all of the
information in the document regarding the product remains unchanged and matches the
product ordered. For further information please contact mlsbranding@sial.com.
Version: 6.1
Revision Date: 06/14/2019
Aldrich – GF18024511
The life science business of Merck KGaA, Darmstadt, Germany
operates as MilliporeSigma in the US and Canada
Print Date: 08/21/2021
Page 9 of 9
SAFETY DATA SHEET
Version 8.0
Revision Date 11/03/2020
Print Date 08/17/2021
SECTION 1: Identification of the substance/mixture and of the company/undertaking
1.1
1.2
Product identifiers
:
Product Number
Brand
CAS-No.
: 1457403
: US Pharmacopeia
: 26159-34-2
Relevant identified uses of the substance or mixture and uses advised against
Identified uses
1.3
1.4
Naproxen Sodium, United States
Pharmacopeia (USP) Reference Standard
Product name
: Laboratory chemicals, Synthesis of substances
Details of the supplier of the safety data sheet
Company
: Sigma-Aldrich Inc.
3050 SPRUCE ST
ST. LOUIS MO 63103
UNITED STATES
Telephone
Fax
: +1 314 771-5765
: +1 800 325-5052
Emergency telephone
Emergency Phone #
: 800-424-9300 CHEMTREC (USA) +1-703527-3887 CHEMTREC (International) 24
Hours/day; 7 Days/week
SECTION 2: Hazards identification
2.1
Classification of the substance or mixture
GHS Classification in accordance with 29 CFR 1910 (OSHA HCS)
Acute toxicity, Oral (Category 4), H302
Reproductive toxicity (Category 1A), H360
For the full text of the H-Statements mentioned in this Section, see Section 16.
2.2
GHS Label elements, including precautionary statements
Pictogram
Signal word
Danger
Hazard statement(s)
H302
Harmful if swallowed.
US Pharmacopeia – 1457403
The life science business of Merck KGaA, Darmstadt, Germany
operates as MilliporeSigma in the US and Canada
Page 1 of 9
H360
May damage fertility or the unborn child.
Precautionary statement(s)
P201
Obtain special instructions before use.
P202
Do not handle until all safety precautions have been read and
understood.
P264
Wash skin thoroughly after handling.
P270
Do not eat, drink or smoke when using this product.
P280
Wear protective gloves/ protective clothing/ eye protection/ face
protection.
P301 + P312 + P330
IF SWALLOWED: Call a POISON CENTER/ doctor if you feel
unwell. Rinse mouth.
P308 + P313
IF exposed or concerned: Get medical advice/ attention.
P405
Store locked up.
P501
Dispose of contents/ container to an approved waste disposal
plant.
2.3
Hazards not otherwise classified (HNOC) or not covered by GHS – none
SECTION 3: Composition/information on ingredients
3.1
Substances
Molecular weight
CAS-No.
: 252.2 g/mol
: 26159-34-2
Component
Classification
(S)-6-Methoxy-α-methyl-2-naphthaleneacetic acid sodium salt
Acute Tox. 4; Repr. 1A;
H302, H360
Concentration